FIG. 4.

Screening of clones for complex I defect. Crude membrane extracts (120 μg of protein solubilized with 1.5% [wt/wt] dodecyl-β-d-maltoside) of Chlamydomonas strains were loaded on a BN gel. After electrophoresis, the gel was stained for NADH dehydrogenase activity of complex I using NBT as an electron acceptor. Mitochondrial complex I is detected at 950 kDa. The two lower-molecular-mass bands (about 750 and 700 kDa) are shown as loading controls and correspond to green bands of photosystem II and I, respectively, associated with light harvesting complexes (71).