FIG. 2.

In situ RCA of E. coli O157:H7 cells carrying the gfp gene added to river water samples. Total bacteria (A), E. coli O157:H7 cells (B), and cells carrying gfp (C) were visualized under UV, blue, and green excitation, respectively. E. coli O157:H7 cells carrying the gfp gene were fixed with 2% formaldehyde after 2 h of incubation in river water taken at Juhachijo, Osaka, Japan. E. coli O157:H7 cells were stained with FITC-labeled anti-E. coli O157:H7 antibody. Cells carrying gfp were detected by in situ RCA targeting of the gene. Scale bars, 5 μm.