TABLE 1.

Proteins identified in B. anthracis spores by using MALDI-TOF MS^a

Spot no.	Accession no.	Theoretical mass (Da)	Mowse score	% Coverage	No. of peptides matching	Protein identification
1	gi 30263563	99,035	156	24	18	Aconitate hydratase 1
2	gi 30260298	76,333	145	27	18	Translation elongation factor G
3	gi 30263811	78,204	65	16	10	Polyribonucleotide nucleotidyltransferase
4	gi 30264669	62,234	104	23	10	Pyruvate kinase
5	gi 30261021	91,361	141	32	19	S-layer protein EA1
6	gi 30258721	87,226	92	17	10	5-Methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
7	gi 30264041	49,452	91	24	9	Dihydrolipoamide dehydrogenase (E3)
8	gi 30261304	70,361	60	16	7	Oligoendopeptidase F
9	gi 30265362	59,749	71	19	7	CTP synthase
10	gi 30264385	65,764	167	21	21	Chaperone protein dnaK
11	gi 30263628	72,408	103	23	14	Transketolase
12	gi 30260443	57,430	175	50	25	Chaperonin, 60 kDa
13	gi 30260481	56,224	172	41	16	Delta-1-pyrroline-5-carboxylate dehydrogenase, putative
14	gi 30260755	61,006	175	31	15	Neutral protease
15	gi 30264541	47,212	97	36	13	Trigger factor
16	gi 30264163	46,223	54	25	9	Pyrimidine-nucleoside phosphorylase
17	gi 30265328	51,192	153	49	22	ATP synthase F1, beta subunit
18	gi 30264042	44,900	72	36	12	Dihydrolipoamide acetyltransferase (E2)
19	gi 30264939	50,344	56	32	9	Phosphoglucose isomerase
20	gi 30260491	52,294	120	39	13	Glutamyl-tRNA(Gln) amidotransferase, A subunit
21	gi 30263502	53,742	113	44	16	Aldehyde dehydrogenase
22	gi 30265485	47,422	83	20	6	Adenylosuccinate synthetase
23	gi 30265161	46,417	188	41	14	Enolase
24	gi 30260328	34,933	68	21	7	DNA-directed RNA polymerase, alpha subunit
25	gi 30253620	42,937	168	61	20	Translation elongation factor Tu
26	gi 30264043	35,228	150	56	13	Pyruvate dehydrogenase complex E1 component, beta subunit
27	gi 30264240	39,850	155	35	13	Leucine dehydrogenase
28	gi 30263830	32,434	57	21	6	Translation elongation factor Ts
29	gi 30260428	43,660	152	30	14	Alanine racemase
30	gi 30265339	45,109	61	11	4	Serine hydroxymethyltransferase
31	gi 30262916	40,023	139	45	17	Chorismate mutase
32	gi 30265022	29,057	60	27	9	ABC transporter, ATP-binding protein
33	gi 30264236	35,790	65	31	9	3-Methyl-2-oxobutanoate dehydrogenase, beta subunit
34	gi 30265359	30,672	95	37	10	Fructose-bisphosphate aldolase, class II
35	gi 30263337	24,006	79	37	7	Transaldolase, putative
36	gi 30260259	32,918	170	65	26	Cysteine synthase A
37	gi 30261955	34,786	81	26	11	l-Lactate dehydrogenase
38	gi 30263838	31,211	63	33	8	Succinyl-CoA synthase, alpha subunit
39	gi 30265165	35,824	117	43	11	Glyceraldehyde 3-phosphate dehydrogenase
40	gi 30264044	41,440	78	21	8	Pyruvate dehydrogenase (E1)
41	gi 30264763	22,323	52	20	4	tRNA binding domain protein, putative
42	gi 30264588	34,340	83	46	13	Electron transfer flavoprotein, alpha subunit
43	gi 30265392	32,389	95	39	12	Agmatinase, putative
44	gi 30264444	25,253	70	54	11	MTA
45	gi 30265465	24,021	120	48	9	Superoxide dismutase, Mn
46	gi 30263350	22,755	150	52	12	Hypothetical protein BA3444
47	gi 30264918	29,691	73	36	10	Naphthoate synthase
48	gi 30261145	31,405	63	32	6	Hypothetical protein BA1021
49	gi 30265367	31,324	61	14	5	3-Hydroxyacyl-CoA dehydrogenase
50	gi 30264875	25,288	58	47	9	Hypothetical protein BA5061
51	gi 30260567	21,101	95	50	8	Tellurium resistance protein
52	gi 30264449	17,437	74	56	6	Transcription elongation factor GreA
53	gi 30261332	17,330	68	56	5	Hypothetical protein BA1237
54	gi 30260515	20,719	139	68	15	Alkyl hydroperoxide reductase, subunit C
55	gi 30260290	18,036	149	66	14	Ribosomal protein L10
56	gi 30263075	18,449	150	52	12	Hypothetical protein BA3129
57	gi 30263828	20,679	109	51	8	Ribosome recycling factor
58	gi 30264160	24,611	71	50	6	Hypothetical protein BA4304
59	gi 30262026	30,100	59	30	6	NH3-dependent NAD+ synthetase
60	gi 30263790	9,338	82	78	5	Hypothetical protein BA3920
61	gi 30265409	34,726	70	20	6	Phosphate acetyltransferase
62	gi 30261415	20,412	215	88	20	PhaP protein
63	gi 30265163	26,437	92	42	12	Triosephosphate isomerase
64	gi 30260245	19,691	76	35	8	Stage V sporulation protein T
65	gi 30260944	21,649	95	44	9	CotJC protein
66	gi 30265393	31,142	130	33	11	Spermidine synthase
67	gi 30265176	21,390	182	53	19	ATP-dependent Clp protease, proteolytic subunit ClpP
68	gi 30264164	29,533	83	28	10	Purine nucleoside phosphorylase
69	gi 30263352	31,828	82	22	7	Oxidoreductase, aldo
70	gi 30263832	28,756	84	25	8	Transcriptional regulator CodY

^aThe spot number identifies the corresponding protein spot on the 2-DE gel as shown in Fig. 1.