TABLE 3.
PCR egc characterization of strains showing SEI-1/SEG-2 weak amplification bands
| Strain | Sourcea | PCR resultb
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| sem-segc | seψ | seu | seo | sem |
sei
|
sen |
seg
|
||||
| SEI-1 SEI-2 | SEI-1 SEI-4 | SEG-1 SEG-2 | SEG-1 SEG-4 | ||||||||
| 10 | DSAT | (+) | + | −e | + | + | − | + | + | − | + |
| 107 | DSAT | (+)d | + | −e | + | + | + | + | + | − | + |
a
DSAT, Dipartimento di Scienze degli Alimenti Università degli Studi di Teramo, Italy (kindly provided by A. Ianieri).
b
If not specified, PCR results are based on the use of previously published primer pairs (8, 19, 25).
c
PCR using the primer pair SEI-1 (targeting sem) and SEG-2 (targeting seg) covering a 3,375-bp fragment of the egc cluster.
d
(+), weak PCR amplification result.
e
The size of the amplification band was other than expected.