Melting curves of the 5.8S rDNA/ITS product of seven Naegleria species: N. fowleri (a), N. lovaniensis (b), N. italica (c), N. australiensis (d), N. gruberi (e), N. byersi (f) N. carteri (g), and Willaertia magna (h). Diagnostic features include the number and position of peaks and their relative heights.