TABLE 1.

Real-time quantitative PCR analysis of changes in Geothrix fermentans gene copy number compared with array intensity

Sample sourcea	Replicate no.	Normalized array intensityd	No. of 16S rRNA gene copies g−1
Area 2	1	133b	NDc
	2	119b	ND
	3	135b	ND
Reduction	1	6,309	1.7 × 106
	2	6,295	3.3 × 106
	3	6,122	4.7 × 106
Oxidation	1	5,497	2.7 × 106
	2	6,054	4.0 × 106
	3	6,433	9.5 × 106

^aArea 2 indicates the original sediment prior to column packing, reduction indicates samples taken during the net uranium reduction phase, and oxidation indicates samples taken during the net uranium oxidation phase.

^bThe Geothrix fermentans probe set was below the positive fraction threshold of 0.90 (90% of probe pairs must pass) in these samples and therefore is considered not detected by the array.

^cND, none detected.

^dArray intensities are expressed in arbitrary fluorescence units.