FIG. 4.

(A) Gene organization around the pce gene cluster of wt strain Y51 and SD and LD variants. Primers for RT-PCR used in this study are depicted as arrowheads. The EcoRI sites are shown by vertical arrows. The mRNA transcripts detected by RT-PCR are shown as solid bars. (B) RT-PCR analyses for total RNA from wt strain Y51. RT-PCR was done targeting intergenic regions between pceA and pceB (primers 1 and 2), pceB and pceC (primers 3 and 4), pceC and pceT (primers 5 and 6), and pceA and pceC (primers 1 and 4), respectively. Lane 1, genomic DNA as the template; lane 2, RNA as the template, no RT; lane 3, RNA as the template, with RT. The sizes of RT products are indicated to the left of the blots. (C) Northern blot analyses using pceA and pceT as the probes for wt strain Y51 (W) and SD variant (S). Total RNAs from wt Y51 and SD variant were electrophoresed with RNA size markers (leftmost blot).