Figure 6.
Light-induced germination of zygotes generated from gametes with reduced phototropin levels. (A) Phototropin levels in gametes used for the generation of zygotes. (B) Germination of zygotes induced by irradiation with white light of a fluence rate of 30 μmol⋅m−2⋅s−1. Zygotes were generated on plates following the protocol given in Materials and Methods. The percentage of zygotes germinated was calculated by dividing the number of zygotes that formed colonies by the total number of zygotes (germinated and nongerminated). Zygotes generated by the mating of two wild-type strains, of wild type and the RNAi20 strain, and of two RNAi20 strains were assayed. The data given represent the average from five independent experiments.