TABLE 1.
OTR (mg/liter/h) | Fermentation progress | Product yield (g of product formed or oxygen consumed/g glucose consumed)a,b
|
Amt of acetoin converted in 2,3-butanediol (%)d | Specific ratea,e
|
|||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Oxygenc | Ethanol | Glycerol | Pyruvate | Acetoin | 2,3-Butanediol | Glucose consumption (mg/108 cells/h) | Ethanol formation (mg/108 cells/h) | Oxygen uptake (μg/108 cells/h) | |||
20 | 0.5 | 7.9 (0.0) | 0.40 (0.00) | 0.032 (0.01) | 0.004 (0.00) | 0.063 (0.005) | 0.011 (0.000) | 16 | 1.42 (0.02) | 0.57 (0.01) | 11.2 (0.03) |
14 | 0.5 | 8.1 | 0.40 | 0.035 | 0.005 | 0.061 | 0.010 | 14 | 1.39 | 0.55 | 10.7 |
11 | 0.5 | 7.8 | 0.39 | 0.032 | 0.006 | 0.059 | 0.011 | 16 | 1.41 | 0.58 | 10.9 |
9 | 0.5 | 4.1 | 0.46 | 0.033 | 0.005 | 0.045 | 0.008 | 15 | 2.35 | 1.12 | 8.44 |
0.97 | 4.4 | 0.47 | 0.020 | 0.001 | 0.044 | 0.008 | 16 | ||||
5 | 0.4 | 1.6 | 0.46 | 0.034 | 0.006 | 0.031 | 0.006 | 16 | 2.07 | 1.01 | 2.79 |
1 | 1.6 | 0.47 | 0.021 | 0.001 | 0.026 | 0.010 | 28 | ||||
Anaerobic | 0.5 | 0 | 0.48 (0.000) | 0.051 (0.001) | 0.002 (0.000) | 0 | 0.005 (0.001) | 100 | 1.51 (0.01) | 0.75 (0.02) | 0 |
1 | 0 | 0.49 (0.000) | 0.032 | 0.001 (0.000) | 0 | 0.004 (0.000) | 100 |
Values in parentheses are standard deviations calculated from two independent experiments.
Only some metabolites are shown. The carbon recovery (including acetate, acetaldehyde, succinate, 2-oxoglutarate, 2-hydroxyglutarate, CO2, and biomass) was between 105 and 115%.
Values are in mg of oxygen consumed per g of glucose consumed.
Calculated as 100 × {[2,3-butanediol]/([acetoin] + [2,3-butanediol])}.
Average of values during the stationary phase.