TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| Strains | ||
| X. oryzae pv. oryzicola | ||
| RS105 | Wild type, Rifr | This lab |
| RABC | prhA::Cm, prhA mutant, Rifr Cmr | This study |
| RCX | hrpX::Cm, hrpX mutant, Rifr Cmr | This study |
| RCG | hrpG::Cm, hrpG mutant, Rifr Cmr | This study |
| RFBC | hrpF::Cm, hrpF mutant, Rifr Cmr | This study |
| M55 | hrpX mutant generated by chemical mutagenesis | 14 |
| M1005 | hrpG mutant generated by chemical mutagenesis | 14 |
| Escherichia coli | ||
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 deoR recA1 hsdR17(rK− mK+) phoA supE44 λ−thi-1 gyrA96 relA1 | Clontech |
| S17-1 | Tra+recA Spr | 48 |
| BL21(DE3) | F−ompT hsdB(rB− mB−) gal dcm (DES) | Novagene |
| Bhpa1 | Conjugant of BL21(DE3) with plasmid pETHpa1 | This study |
| Plasmids | ||
| pUFR034 | Kmr, IncW, Mob(p), Mob+, LacZa+, PK2 replicon, cosmid | 17 |
| pBlueScript II KS(−) | Phagemid, pUC derivative, Apr | Stratagene |
| pBC SK | pUC ori, Cmr | Stratagene |
| pUC18 | pUC ori, Apr | This lab |
| pMD-18-T | Apr, T-easy vector for PCR products | TaKaRa |
| p6 | Kmr, positive hrp gene clone screened from X. oryzae pv. oryzicola genomic library | This study |
| pPK12.3 | Kmr, a 12.3-kb fragment containing the hrpG and hrpX operons screened from X. oryzae pv. oryzicola genomic library | 14 |
| pA1 | Kmr, positive hrp gene clone screened from X. oryzae pv. oryzicola genomic library | This study |
| pA1-E2 | Apr, a 5.1-kb EcoRI fragment from pA1 in pUC18 | This study |
| pHA1.5 | Apr, a 1.5-kb hrpA of X. oryzae pv. oryzae ligated into pUC18 as a probe for screening the genomic library of X. oryzae pv. oryzicola | This study |
| pHF1.62 | Apr, a 1.62-kb hrpF fragment of X. oryzae pv. oryzae ligated into pBluescript KS(−) as a probe for screening the genomic library of X. oryzae pv. oryzicola | This study |
| p6-28 | Apr, a 9,611-bp EcoRI fragment from p6 ligated into pBluescript KS(−) | This study |
| p6-7 | Apr, a 5,071-bp EcoRI fragment from p6 ligated into pBluescript KS(−) | This study |
| p6-8 | Apr, a 7,808-bp EcoRI fragment from p6 ligated into pBluescript KS(−) | This study |
| p685 | Apr, a 685-bp EcoRI fragment from p6 ligated into pMD-18-T vector | This study |
| p6-3 | Apr, a 1,996-bp EcoRI fragment from p6 ligated into pBluescript KS(−) | This study |
| pBSK579 | Apr, a 579-bp EcoRI-KpnI fragment from p6 ligated into pBluescript KS(−) | This study |
| p6121-2 | Apr, a 6,381-bp KpnI fragment from p6 ligated into pBluescript KS(−) | This study |
| pHpa21 | Apr, a 1,356-bp fragment containing the hap2 and hpa1 genes ligated into pMD-18-T | This study |
| p61 | Kmr, a 2,163-bp prhA gene from pHA1.5 ligated in pUFR034 | This study |
| phrA2.1 | Apr, PCR product of a 2,163-bp prhA gene ligated into pBluescript | This study |
| pABC | Cmr, a 112-bp PstI-SacII fragment from phrA2.1 ligated in pBC SK(−) | This study |
| pEThpa1 | Apr, a 414-bp hpa1 gene of X. oryzae pv. oryzicola cloned into pET21a | This study |
| pUhpa1 | Kmr, a 614-bp hpa1 gene plus the promoter region together with the fragment encoding a His6 tag cloned into pUFR034 | This study |
| pBCX147 | Cmr, a 147-bp PstI-KpnI fragment from the hrpX gene ligated in pBC SK(−) | This study |
| pBCG121 | Cmr, a 121-bp EcoRI-SacI fragment from the hrpG gene into pBC SK(−) | This study |
| pFBC | Cmr, a 351-bp BamHI-PstI fragment from p6-3 linked into pBC SK(-) | This study |
| pHrpF | Kmr, a 2,409-bp hrpF gene by PCR linked into pUFR034 | This study |
a
Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Rifr, rifampin resistance; Spr, spectinomycin resistance.