TABLE 2.
Primers used in this study
| Primer | Sequence (5′→3′)a | Purpose |
|---|---|---|
| prhA-F1 | CTGAATTCATGTCTGCGACCTTCCC | To amplify the prhA gene from X. oryzae pv. oryzicola |
| prhA-R1 | AGGAATTCCTAGAAATCCACCTGC | |
| hpa1-F1 | CATATGAATTCTTTGAACACACAATTC | To express the hpa1 gene in E. coli |
| hpa1-R1 | CTCGAGCTGCATCGATCCGCTGTCGTTCG | |
| hpa1-F2 | GGATCCGATCTGTTATCGATCCTAAAAAATTTTCCAC | To express the hpa1 gene together with a hexahistidine tag in X. oryzae pv. oryzicola |
| hpa1-R2 | GGTACCTCAGTGGTGGTGGTGGTGGTGCTGCATCGATCCGCTGTCGTTCG | |
| hrpA-F1 | CGGATCCACTTAACGGGCAAGAAAAAAG | To amplify the hrpA gene as a probe for screening for hrp clones from an X. oryzae pv. oryzicola genomic library |
| hrpA-R1 | TGGATCCGGTACCGGGTCTGTCAAAGATTC | |
| hrpF-F1 | GAAGGATCCGCGGTTGTTCTTCGCCATC | As a probe for screening for hrp clones from an X. oryzae pv. oryzicola genomic library |
| hrpF-R1 | TGCAAGCTTGGAGGCACGTTCATACGAACG | |
| hrpF-F2 | GGTACCATGTCGCTCAACATGCTTTCTAC | To express the hrpF gene in the hrpF mutant of X. oryzae pv. oryzicola |
| hrpF-R2 | GGTACCTTATCTGCGACGTATCCTGACATTG | |
| hrpX-F | CTGGATCCATGATCCTTTCGACCTACTTTG | To amplify the hrpX gene from X. oryzae pv. oryzicola |
| hrpX-R | ATGGATCCTTACCGTTGCAAGGTT | |
| hrpG-F | GGATCCATGAACATCCCTTGCCCCCTTG | To amplify the hrpG gene from X. oryzae pv. oryzicola |
| hrpG-R | GGATCCTCAGCAGGCGGCTGTGCGATG | |
| hpa2-F | CTATTCACCAATCACACCAC | For cloning the region incorporating the hpa2 to hpa1 genes from X. oryzae pv. oryzicola |
| hpa1-R | TTACTGCATCGATGCGCT |
a
Restriction sites are underlined.