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HPLC analysis of carotenoid products in E. coli cells expressing wild-type (WT) CrtW and M99I, M99V, and L175M mutants. E. coli cells contained the plasmid pSUBCMW and the reporting construct pBADZX (Table 1). Pigments were extracted from the cells after 8 h of growth. The carotenoids identified were astaxanthin (Ax), adonixanthin (Adx), and zeaxanthin (Zx).
