FIG. 1.

Purified DNA on agarose gel from supernatant (free DNA) (sup) and pellet (cell DNA) fractions of P. freudenreichii ISU P59 cultivated in SLB broth at 30°C at 2 and 14 days (a) and absorbance measurements (A₆₀₀) for the 5-week incubation period (b). The samples were separated for 1 h (90 V) on an agarose gel (0.8%) containing ethidium bromide (1 μg/ml). A DNA ladder (MBI Fermentas) ranging from 1,500 bp to 10,000 bp is shown. Error bars represent standard deviations for at least four replicates.