TABLE 1.
Species-specific DNA compositiona
| Bacterial strain and probe | Incubation time for indicated DNA type
|
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 h
|
12 h
|
24 h
|
48 h
|
7 d
|
14 d
|
5 wk
|
||||||||
| Cellc | Free | Cell | Free | Cell | Free | Cell | Free | Cell | Free | Cell | Free | Cellc | Free | |
| L. lactis subsp. lactis INF L2 | ||||||||||||||
| LCL | 71 ± 8b | - | 77 ± 30b | 70 ± 30 | 77 ± 29b | 73 ± 29 | 77 ± 30b | 73 ± 30 | 80 ± 20b | 76 ± 20 | 80 ± 27b | 77 ± 27 | 66 ± 20 | 72 ± 20 |
| PAB#3 | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| PAB#4 | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| PAB#5 | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| P. acidipropionici ATCC 4965 | ||||||||||||||
| LCL | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| PAB#3 | 69 ± 19b | - | 79 ± 14b | - | 81 ± 12b | - | 81 ± 6b | 75 ± 7 | 80 ± 9b | 67 ± 11 | 87 ± 22b | 73 ± 19 | 75 ± 14 | 81 ± 4 |
| PAB#4 | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| PAB#5 | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| P. freudenreichii ISU P59 | ||||||||||||||
| LCL | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| PAB#3 | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| PAB#4 | 52 ± 5b | - | 70 ± 13b | - | 73 ± 18b | - | 73 ± 3b | 57 ± 15 | 72 ± 2b | 66 ± 1 | 49 ± 7b,c | 72 ± 22 | 50 ± 7 | 68 ± 10 |
| PAB#5 | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| P. jensenii INF P303 | ||||||||||||||
| LCL | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| PAB#3 | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| PAB#4 | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| PAB#5 | 37 ± 10b | - | 54 ± 13b | - | 62 ± 4b | - | 65 ± 9b | - | 59 ± 4b | 49 ± 6 | 62 ± 19b | 54 ± 8 | 52 ± 6 | 50 ± 8 |
| MIX | ||||||||||||||
| LCL | 34 ± 4b | - | 66 ± 6b | 34 ± 3 | 48 ± 12b | 51 ± 7 | 29 ± 2b | 18 ± 2 | 43 ± 6b | 20 ± 4 | 43 ± 9b | 20 ± 4 | 28 ± 6 | 20 ± 1 |
| PAB#3 | 27 ± 6b | - | 18 ± 1b | - | 23 ± 2b | - | 35 ± 8b | 44 ± 1 | 33 ± 8b | 18 ± 3 | 26 ± 6b | 25 ± 5 | 25 ± 4 | 34 ± 13 |
| PAB#4 | - | - | - | - | 21 ± 9b | - | 24 ± 2b | 21 ± 8 | 11 ± 8b | 49 ± 15 | 13 ± 0b | 40 ± 14 | 15 ± 4 | 35 ± 10 |
| PAB#5 | - | - | - | - | - | - | 13 ± 8b | - | 13 ± 4b | 15 ± 7 | 18 ± 4b | 15 ± 7 | 32 ± 4 | 12 ± 4 |
| PAB | NA | NA | NA | NA | ||||||||||
| LCL | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| PAB#3 | 48 ± 1 | - | 56 ± 10 | - | 58 ± 10 | - | 48 ± 14 | 34 | 50 ± 13 | 33 | 53 ± 12 | 40 | 55 ± 9 | 51 |
| PAB#4 | - | - | - | - | 21 ± 3 | - | 22 ± 6 | 31 | 19 ± 3 | 43 | 18 ± 1 | 32 | 23 ± 8 | 22 |
| PAB#5 | - | - | - | - | - | - | 22 ± 4 | - | 22 ± 1 | 15 | 21 ± 4 | 22 | 18 ± 6 | 20 |
a
Relative DNA fractions for the different bacteria are shown. Calculations were done as previously described (44). Probe abbreviations: LCL, L. lactis; PAB#3, P. acidipropionici; PAB#4, P. freudenreichii; PAB#5, P. jensenii. Standard deviations for two or three independent replicates are shown. Abbreviations: cell, cell DNA; free, free DNA; MIX, mixed sample of all the strains; PAB, coculture of the propionibacteria; -, signal less than background threshold; NA, no parallels are available.
b
Data from Treimo et al. (44).
c
Weak signals due to low cell content.