Thin-layer chromatography of hydrolysis products from agarose (A) and from neoagarooligosaccharides and some polysaccharides (B) with rAgaC. Gal, galactose; DP2, DP4, DP6, DP8, and DP10, degrees of polymerization of neoagarooligosaccharides; S, standard neoagarooligosaccharides; E, rAgaC. Lanes 1, 3, 5, 7, and 9, DP2, DP4, DP6, DP8, and DP10, respectively; lanes 2, 4, 6, 8, and 10, hydrolysis products from DP2 to DP10, respectively; lanes 11, 13, 15, and 17, agarose, agar, porphyran, and κ-carrageenan, respectively; lanes 12, 14, 16, and 18, hydrolysis products from agarose, agar, porphyran, and κ-carrageenan, respectively.