TABLE 1.
Antibacterial activities of nukacin ISK-1 and its fragments and mutants along with their inhibitory effects on nukacin ISK-1 and each other
| Nukacin ISK-1 or derivative | MIC (μM)a
|
||
|---|---|---|---|
| L. sakei | P. pentosaceus | L. mesenteroides | |
| Nukacin ISK-1 (control) | 0.26 | 0.52 | 0.26 |
| +2K nukacin ISK-1 | 0.26 | 0.52 | 0.26 |
| Nukacin ISK-1 + tail region | 0.26 | 0.52 | 0.26 |
| Nukacin ISK-1 + ring region | 0.26 | 0.52 | 0.26 |
| Nukacin ISK-1 + K1-3A nukacin ISK-1 | 1.04 | 2.08 | 1.04 |
| Nukacin ISK-1 + nukacin4-27 | 1.04 | 2.08 | 1.04 |
| K1-3A nukacin ISK-1 | 8.32 | 16.64 | 8.32 |
| Nukacin4-27 | 8.32 | 16.64 | 8.32 |
| Tail region (nukacin1-7) | ND | ND | ND |
| Ring region (nukacin7-27) | ND | ND | ND |
| Tail region + ring region | ND | ND | ND |
a
Antibacterial activity was determined at least twice for each derivative. Results are shown for L. sakei subsp. sakei JCM 1157T, P. pentosaceus JCM 5885, and L. mesenteroides subsp. mesenteroides JCM 6124T. ND, not detected.