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. 2006 Sep;72(9):6136–6141. doi: 10.1128/AEM.00341-06

TABLE 1.

Primer sequences developed for real-time PCR of type A and B V. vulnificus

Primer Sequence Positiona Amplicon size (bp) Type amplified
VvAF1b CATGATAGCTTCGGCTCAA 171-190 285 A
VvAR1c CAGCACTCCTTCCACCATCAC 435-455
VvBF1b GCCTACGGGCCAAAGAGG 177-194 841 B
VvBR1c GTCGCCTCTGCGTCCAC 1001-1017
a

Position refers to the nucleotide numbers for the 16S rRNA sequences of X76333 (type A) and X76334 (type B) in GenBank.

b

Primer derived from M. Vickery et al (42).

c

Primer derived from this study.