TABLE 2.
Comparison of yields and rates for ANAS and published dechlorinating enrichment cultures
| Culture (substrate) | Mean ± SDa
|
Source or reference | |||
|---|---|---|---|---|---|
| No. of copies/μmol of Cl− (107)
|
Rateb (10−10) | ||||
| Deh. 16Sc | vcrA | tceA | |||
| ANAS (VC) | 1.3 ± 0.3 | 1.3 ± 0.3 | 8.6 ± 1.32 | This study | |
| ANAS (cDCE) | 1.1 ± 0.1 | 1.2 ± 0.1* | 1.2 ± 0.11 | 7.6 ± 0.4† | This study |
| ANAS (TCE) | 1.4 ± 0.4 | 1.3 ± 0.4* | 1.6 ± 0.41 | 2.3 ± 0.6† | This study |
| 195 (PCE) | 230 ± 20‡ | 2.2 ± 0.3‡ | 21 | ||
| BAV1 (VC) | 6.3 ± 0.3 | 9 | |||
| VS (VC) | 46 ± 3 | 7.8 | 3 | ||
| VS (TCE) | 58 ± 8 | 7.8 | 3 | ||
| KB1 (VC) | 56 ± 14 | 2.2 ± 0.4 | 9 | ||
| KB1 (TCE) | 3.0 ± 0.3 | 9 | |||
| GT (VC) | 25 ± 2 | 10.1 | 30 | ||
a
*, yields for vcrA and tceA genes are calculated based on the assumption that vcrA is responsible for VC dechlorination and tceA is responsible for TCE and cDCE dechlorination. †, rates were calculated from the interval of maximum solvent dechlorination observed during culture growth. ‡, yields and rates for D. ethenogenes 195 were calculated by applying the protein-to-cell conversion factor of 4.2 × 10−15 g/cell from Duhamel et al. (9) to published results of Maymo-Gatell et al. (21).
b
Expressed as μmol of Cl−/(copies of 16S gene · day).
c
Deh. 16S, Dehalococcoides 16S rRNA gene.