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GFP expression from segregants containing Ll.ltrB::GFP in tra904 alleles. The “0.1” designation after the strain name indicates that the intron delivery plasmid was removed and the vector pMSP3535 was introduced as a source of nisRK required for nisin-inducible expression of GFP. The number above each column indicates the number of tra904 copies invaded. The “ON” and “120G” designations represent cultures grown overnight and for 120 generations, respectively.
