TABLE 1.
Bacterial strains assayed by molecular-beacon real-time PCR
| Serogroup and strain | Sourcea (origin) | Detectionb of the indicated gene by:
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Single PCR (1 × 103 CFU)
|
Multiplex PCR (1 × 105 to 5 CFU)c
|
||||||||
| rtxA | epsM | ompW | tcpA | rtxA | epsM | ompW | tcpA | ||
| V. cholerae O1 classical | |||||||||
| 11966 | A (Bangladesh, 1987) | + | + | + | − | + | + | + | − |
| AA14041 | A (Bangladesh, 1985) | + | + | + | − | + | + | + | − |
| Z17561 | A (Bangladesh, 1985) | + | + | + | − | + | + | + | − |
| Z17561 tcpA::kan (tcpA mutant) | A (University of Adelaide) | + | + | + | − | + | + | + | − |
| 0162 | C (India) | + | + | + | − | ||||
| 162 | C (India) | + | + | + | − | ||||
| 569B | C (Unknown) | + | + | + | − | ||||
| 569B-685RNM | C (Unknown) | + | + | + | − | ||||
| 35A3 | C (Unknown) | + | + | + | − | ||||
| 111-V585R | C (Unknown) | + | + | + | − | ||||
| 95 | C (India) | + | + | + | − | ||||
| V. cholerae El Tor | |||||||||
| N16961 | A (Unknown) | + | + | + | + | + | + | + | + |
| AA13993 | A (Bangladesh, 1985) | + | + | + | + | + | + | + | + |
| H1 | A (India, 1985) | + | + | + | + | + | + | + | + |
| H1 tcpA::kan (tcpA mutant) | A (University of Adelaide) | + | + | + | − | + | + | + | − |
| HP-51-1 | C (Thailand, 1972) | + | + | + | + | ||||
| BRL 7738 | C (1966) | + | + | + | + | ||||
| N107 | C (Unknown) | + | + | + | + | ||||
| VB1961 | C (Unknown) | + | + | + | + | ||||
| I-816 | C (Unknown) | + | + | + | + | ||||
| V. cholerae O139 | |||||||||
| AI-1838 | A (Bangladesh, 1993) | + | + | + | + | + | + | + | + |
| AI-1854 | A (Bangladesh, 1993) | + | + | + | + | + | + | + | + |
| AI-1855 | A (Bangladesh, 1993) | + | + | + | + | + | + | + | + |
| V. cholerae non-O1 | |||||||||
| V. cholerae non-O1 | B (Fairfield Hospital, Australia) | + | + | + | − | ||||
| H II (nonagglutinating)d | C (Hong Kong, 1963) | + | + | + | − | ||||
| Other Vibrio spp. | |||||||||
| V. anguillarum ATCC 19246e | ATCC | − | − | − | − | ||||
| V. alginolyticus | B (MDU, Australia) | − | − | − | − | ||||
| V. alginolyticus ATCC 17749 | ATCC | − | − | − | − | ||||
| V. campbellii ATCC 25920e | ATCC | − | − | − | − | ||||
| V. fischerie | B (Microtox kit strain 2000) | − | − | − | − | ||||
| V. fluvialis | A (Unknown) | − | − | − | − | ||||
| V. harveyi 179e (tentative name) | C (Unknown) | − | − | − | − | ||||
| V. mimicus | A (Unknown) | +/− | − | − | +/− | − | |||
| V. mimicus | B (Fairfield Hospital, Australia) | +/− | − | − | +/− | − | |||
| V. natriegens NCMB 857f | NCMB | − | − | − | − | ||||
| V. pagrus MIC-2e | C (Pagrus auratus) | − | − | − | − | ||||
| V. parahaemolyticus ATCC 17802 | ATCC | − | − | − | − | ||||
| V. parahaemolyticus NCTC10884 | NCTC | − | − | − | − | ||||
| V. parahaemolyticus NCTC10885 | NCTC | − | − | − | − | ||||
| V. vulnificus | B (RCPA, QAP 1995:8:4) | − | − | − | − | ||||
| V. vulnificus C7184 | CDC | − | − | − | − | ||||
| V. vulnificus C7184T | CDC | − | − | − | − | ||||
| Other bacteria | |||||||||
| Bacillus subtilis ATCC 6051f | ATCC | − | − | − | − | ||||
| Enterococcus faecalis 159905660 | Pathcentre, Perth, Australia | − | − | − | − | ||||
| Escherichia coli PA03M55679 | Pathcentre, Perth, Australia | − | − | − | − | ||||
| Klebsiella pneumoniae 106156559 | Pathcentre, Perth, Australia | − | − | − | − | ||||
| Pseudomonas aeruginosa PA03M2615 | Pathcentre, Perth, Australia | − | − | − | − | ||||
| Serratia marcescens 13023f | Pathcentre, Perth, Australia | − | − | − | − | ||||
| Shigella sonnei ATCC 9290 | ATCC | − | − | − | − | ||||
| Staphylococcus aureus ATCC 9144 | ATCC | − | − | − | − | ||||
| Yersinia enterocolitica W22703 | Melbourne University | − | − | − | − | ||||
a
A, Stephen Attridge, University of Adelaide; B, Celia McKenzie, Royal Melbourne Institute of Technology; C, University of New South Wales; MDU, Microbiological Diagnostics Unit; RCPA, Royal College of Pathologists of Australasia Quality Assurance Program.
b
Blank, not tested; +, specific amplified product detected; −, no amplified product detected; +/−, limited product detected.
c
All Vibrio spp. other than V. cholerae and all other bacterial species were assessed using 106 CFU.
d
Serotype unknown.
e
Grown at 25°C.
f
Grown at 30°C.