TABLE 3.
Fourplex detection of V. cholerae from spiked water samples
| Source | Detection of V. choleraea in:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Unprocessed samples (LOD, 103 CFU/reaction)
|
Isolated DNA (LOD, 10 CFU/reaction)
|
|||||||||
| 11966 | N16961 | AI-1839 | Vibriob | − C | 11966 | N16961 | AI-1839 | Vibrio | − C | |
| Sea (Port Phillip Bay, Melbourne, Australia) | − | − | − | +/− | − | + | + | + | +/− | − |
| Estuarine (Yarra River, Melbourne, Australia) | + | + | + | +/− | − | + | + | + | +/− | − |
| River (Plenty River, Victoria, Australia) | + | + | + | +/− | − | + | + | + | +/− | − |
| Dam (South Morang, Victoria, Australia) | + | + | + | +/− | − | + | + | + | +/− | − |
| Commercial spring water | + | + | + | +/− | − | + | + | + | +/− | − |
a
LOD, limit of detection; − C, negative control (unspiked water sample); +, specific amplified product detected; −, no amplified product detected; +/−, limited product detected (ompW only).
b
Vibrio, a mixture of V. fluvialis, V. parahaemolyticus, V. alginolyticus, V. mimicus, and V. vulnificus at 1 × 105 CFU of each strain per PCR.