Figure 4.

eta3 Mutants Exhibit Increased AXR3NT-GUS Stability.

(A) to (H) Wild-type (WT; [A] to [D]) and eta3 ([E] to [H]) seedlings carrying the HS::AXR3NT-GUS reporter construct were heat shocked for 2 h and stained for β-glucuronidase activity after 20- or 40-min incubations at room temperature. IAA (5 μM) was added to the room-temperature incubation where indicated.

(I) AXR2 protein was immunoprecipitated from Columbia (Col), eta3 tir1, eta3, and tir1-1 seedlings labeled with ³⁵S-Met. Precipitations were performed immediately after labeling (t = 0) or after a 15-min chase with medium containing 1 mM Met and 100 μg/mL cycloheximide (t = 15).