Table 1.
Wild Type and Mutant Carotenoid Composition in Leaf Tissue Quantified by HPLC
Genotype | Lutein | Zeinoxanthin | β-Carotene | Neoxanthin | Violaxanthin | Antheraxanthin | Zeaxanthin | β-Xanthophylls | Total Carotenoids |
---|---|---|---|---|---|---|---|---|---|
Ws | 100 ± 9.0 (54)a | — | 31.0 ± 2.5 (17)a | 30.5 ± 5.1 (16)a | 23.1 ± 4.2 (13)a | — | — | 53.6 ± 6.7 (29)a | 184.5 ± 15.0a |
Col | 104.9 ± 6.9 (58)a | — | 31.2 ± 6.7 (17)a | 25.7 ± 2.5 (14)a,b | 20.8 ± 3.9 (11)a,b | — | — | 46.5 ± 4.6 (25)a | 182.5 ± 14.2a |
b1 | 118.5 ± 6.2 (64)b | — | 33.7 ± 5.5 (18)a | 16.9 ± 2.0 (9)c | 16.1 ± 0.6 (9)b | — | — | 33.0 ± 3.5 (18)b | 185.1 ± 7.7a |
b2 | 104.3 ± 3.1 (60)a | — | 26.9 ± 2.9 (15)a | 23.4 ± 2.4 (13)b | 21.2 ± 1.0 (12)a | — | — | 44.6 ± 3.0 (25)a | 175.8 ± 7.8a |
b1 b2 | 130.9 ± 4.7 (74)c | — | 34.3 ± 4.7 (19)a | 3.2 ± 1.1(2)d | 8.4 ± 1.7 (5)c | — | — | 11.6 ± 2.7 (7)c | 176.8 ± 4.1a |
lut1 | 20.4 ± 3.6 (11)d | 35.5 ± 3.1 (20)a | 29.8 ± 6.2 (17)a | 21.9 ± 3.2 (12)b,e | 46.5 ± 4.1 (26)d | 19.2 ± 2.9 (11)a | 5.5 ± 1.0 (3)a | 93.1 ± 6.4 (52)d | 178.8 ± 16.7a |
lut1 b1 | 13.2 ± 1.5 (7)e | 44.1 ± 3.6 (25)b | 40.5 ± 2.6 (23)b | 19.3 ± 1.8 (11)c,e | 35.8 ± 2.1 (20)e | 20.5 ± 3.3 (11)a | 6.3 ± 1.1 (3)a | 81.9 ± 4.6 (46)e | 179.7 ± 6.0a |
lut1 b2 | 11.5 ± 2.2 (6)e | 32.8 ± 5.0 (18)a | 41.1 ± 3.2 (22)b | 20.2 ± 1.3 (11)c,e | 49.1 ± 1.6 (27)d | 22.3 ± 2.7 (12)a | 6.7 ± 1.0 (4)a | 98.3 ± 5.3 (53)d | 183.8 ± 6.9a |
lut1 b1 b2 | 14.1 ± 1.5 (8)e | 88.1 ± 8.6 (50)c | 64.0 ± 5.3 (36)c | 1.6 ± 0.1 (1)f | 8.5 ± 1.2 (5)c | — | — | 10.1 ± 1.7 (6)c | 176.3 ± 6.9a |
The amount of carotenoid is expressed as mmol pigment/mol chlorophyll a + b. Each value is the mean result from six experiments ± SD, with the relative molar percentage of each carotenoid given in parentheses. Values marked with the same letters are not significantly different from each other within a column (Student's t test, P > 0.05).