FIG. 2.

The 18x21-EGFP reporter cassettes can be readily transduced by the SMPU vector and are highly Tax responsive. Four cell lines, MT4, PX1, HeLa, and Jurkat, were selected for transduction by SMPU-18x21-EGFP. MT4 is an HTLV-1-transformed human T-cell line that expresses Tax constitutively. PX1 is a fibroblast cell line derived from an LTR-tax transgenic mouse. PX1 also constitutively produces Tax. HeLa and Jurkat are cell lines unrelated to HTLV-1. Twenty-four hours before transduction, cells were seeded in six-well plates. The same amount of the reporter vector was then added to each cell line individually. EGFP expression was observed 48 h after transduction using an Olympus IX81 inverted fluorescence microscope. Panels on the bottom show fluorescence images of the transduced cells. Panels on the top are corresponding bright-field images. Cell lines used for panels A to F are as labeled. All were transduced with the SMPU-18x21-EGFP reporter. Cell lines in panels D and F were additionally transduced with LV-Tax, a lentivirus vector for HTLV-1 Tax.