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. 2006 Aug;80(15):7769–7774. doi: 10.1128/JVI.02427-05

FIG. 4.

FIG. 4.

FIG. 4.

Abrogation of the block by CSA is independent of capsid- CypA interactions and calcineurin inhibition. (A) Effects of CSA on lentiviral infection in mouse BM cells. Freshly isolated BM cells were infected with HIV-1, SIVmac, and HIV-1 G89V capsid mutant vectors in the presence of CSA (5 μM). Rapamycin (2.5 nM) was used as a control in infection with HIV and SIVmac vectors. GFP-positive populations were analyzed by FACSA 12 days after infection. (B) Freshly isolated BM cells were infected with adenoviral (Ad-GFP) and retroviral (MLV-GFP) vectors in the presence or absence of CSA (5 μM). GFP-positive populations were analyzed by FACSA 5 days after infection. (C) Effects of a nonimmunosuppressive CSA-related drug, Debio-025, on HIV-1 vector infection in mouse BM and spleen-derived T cells. Freshly isolated BM cells and concanavalin A-stimulated spleen-derived T cells from BALB/c mice were infected with HIV-1 vectors in the presence of the indicated concentrations of Debio-025 or CSA. GFP-positive populations were analyzed by FACSA 6 days after infection.