FIG. 3.
Complementation between MT retroviral vectors and the MTnull/STnull A185 mutant virus. NIH 3T3 cells were infected as described in Materials and Methods with retrovirus vectors expressing wild-type or mutant MT or vector control as shown above the lanes (see Table 1 for the mutant naming code). (A and B) Anti-PYV early protein antibody. Cross-reacting cellular bands are marked, as are the LT and MT positions. Results are from 26 h post-retrovirus infection, 6 h post-PYV infection, prior to synthesis of PYV LT (A) or 48 h post-PYV infection (B). (C) Results of blotting with anti-VP1 antibody at 48 hpi.