TABLE 2.

Relative genome amplification patterns of MT mutants in NIH 3T3 cells^a

Virus	Relative amplification
	24 hpi		48 hpi
	−ser	+ser	−ser	+ser
A2	1	1	1	1
Y250F	0.6	0.6	0.3	0.6
Y315F	0.4	0.6	0.4	0.6
Y322F	0.7	0.6	0.6	0.8
Y315/322F	0.4	0.7	0.2	0.7
Y250/315F	0.4	0.6	0.3	0.8
Y250/322F	0.04*	0.1	0.2	0.4
Y3FM	0.4	0.6	0.5	0.7
Y6F	0.1	0.3	0.2	0.2
MT-ter	0.1	0.4	0.2	0.6
1387T	0.3	0.5	0.3	0.6
A185	0.02	0.03	0.02	0.1

^aNIH 3T3 cells were infected with the mutants listed, as described in the legend to Fig. 4, in medium containing 0.5% serum (−ser) or 10% serum (+ser). As shown in Fig. 7, samples were collected at 4 hpi (input), 24 hpi (not shown), and 48 hpi. Quantitation is based on counts in genome size bands as determined by phosphorimager analysis. In each case, the genome amplification was determined as the ratio of output genome level (at 24 or 48 hpi) relative to the level in the input (4 hpi). In the case of the wild type, these values were 46 (24 hpi, without serum), 48 (24 hpi, with serum), 170 (48 hpi, without serum), and 140 (48 hpi, with serum). These values for the wild type were set at 1.0, and the decreases in the mutant relative to wild-type genome yields are shown for the four conditions. *, this sample was underloaded.