TABLE 4.
Effect of stable LT expression on A185 genome amplification with retroviral MT complementationa
| Retrovirus | Complementation factor
|
|||||||
|---|---|---|---|---|---|---|---|---|
| NIH coinfected with:
|
LT-NIH coinfected with:
|
|||||||
| No | Empty | WT-MT | Y6F-MT | No | Empty | WT MT | Y6F MT | |
| Out/In | 3 | 3 | 147 | 15 | ND | 15 | 84 | 28 |
| Complementation | 1 | 1 | 50 | 5 | 1 | 5.6 | 1.9 | |
NIH 3T3 and LT-NIH cells were coinfected with A185 and the retroviral MT wild type and mutants listed, under the conditions described in the legend for Fig. 3. Samples were collected at 48 hpi and analyzed by Southern blotting as described for Fig. 8. The complementation factor induced by MT expression is shown.