TABLE 5.

Maturation potentials of MT⁺/ST⁺, MT^null/ST⁺, and MT^null/ST^null mutants^a

hpi	Assay	Wild type			MT-Ter			Y3FM			Y6F			A185
		C	M	T	C	M	T	C	M	T	C	M	T	C	M	T
48	Virus yieldb	100	4.3	104	18	1.9	20	39	6.2	45	—*	4.5	—*	.5	.53	1.0
	Genome amplificationc	171	NA	171	91	NA	91	330	NA	330	68	NA	71	15.3	NA	15.3
	Maturation ratio			0.6			0.2			0.14			—			0.08
	Normalized maturation ratioe			1.0			0.3			0.2			—			0.1
72	Virus yieldb	75	55	130	26	39	64	55	92	137	20	45	65	1.8	2.3	4.1
	Genome amplificationc	344	NA	344	310	NA	310	1140	NA	1140	411	NA	411	97	NA	97
	Maturation ratiod			0.4			0.2			0.1			0.2			0.04
	Normalized maturation ratioe			1.0			0.5			0.3			0.4			0.1

^aNIH 3T3 cells were infected with the indicated virus strains and cultured in the presence of 10% serum. Cell monolayers (C) and medium (M) were harvested at 4, 48, and 72 hpi. The total (T) represents the sum of C and M. *, no data due to contaminated plates. NA, not applicable.

^bMature live virus yields were quantitated by plaque assays. Numbers shown represent the yield of PFU per infected cell (ratio of the value at 48 or 72 hpi and the input value).

^cViral genomes were quantitated by Southern blotting. Numbers shown represent genome amplification (ratio of the value at 48 or 72 hpi and the value at 4 hpi).

^dThe ratio of mature virus to genome yield, reflecting maturation.

^eMaturation factor normalized so that wild type = 1.0.