Skip to main content
. 2006 Aug;80(15):7287–7294. doi: 10.1128/JVI.00414-06

FIG. 5.

FIG. 5.

Release of SCoV 7a protein in the presence of other viral structural proteins. 7a protein was coexpressed with S protein, M protein, N protein, and E protein in different combinations, as indicated, in 293T cells. Plasmid quantities for this analysis were 1.5 μg of pCAGGS-S, 1.5 μg of pCAGGS-M, 1.5 μg of pCAGGS-N, 0.25 μg of pCAGGS-E, and 1 μg of pCAGGS-7a. Total DNA levels were adjusted by adding pCAGGS. At 48 h after transfection, media were harvested, and released VLPs and/or membrane vesicles were pelleted through a 20% sucrose cushion. Media (Media) and cell lysates (Cell) were analyzed for N protein, M protein, and 7a protein by Western blotting.