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. 2003 Jun;15(6):1347–1359. doi: 10.1105/tpc.009902

Figure 5.

Figure 5.

Sumoylation Modulates ABA-Induced Growth Inhibition.

(A) ABA-mediated root growth inhibition is attenuated in SUMO-overexpressing plants. T2 transgenic plants expressing His-AtSUMO1/2 (AtS1 and AtS2), AtSCE1a cosuppression (co-AtSCE1a), or carrying the empty vector (pBA002 [BA]) were germinated on medium A plates for 3 days, transferred to medium A control or medium A supplemented with 200 mM NaCl, 400 mM mannitol, 10 μM ABA (ABA10), or 50 μM ABA (ABA50), and incubated for 4 additional days before root growth was scored (n = 20). Root length obtained in control medium A was taken to be 100%, and growth in the other conditions was expressed with respect to the control value. Error bars are shown.

(B) ABA-induced growth inhibition is attenuated in SUMO-overexpressing plants and accentuated in plants showing AtSCE1a cosuppression. Three-day-old T2 seedlings were transferred to medium A supplemented with 5 μM ABA and incubated for 24 days before photographs were taken. Bar = 1 cm.