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. 2006 Aug;80(15):7578–7589. doi: 10.1128/JVI.02421-05

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FIG. 4. — TB40E induces the block of chemokine-driven migration in monocytes. Monocyte chemotaxis toward inflammatory (CCL5, CCL2, and CX3CL1) and homeostatic (CCL19 and CXCL12) chemokines was evaluated with a Boyden chamber as described in Materials and Methods. The migration induced by the indicated chemokines (100 ng/ml) was evaluated by assessing each stimulus in triplicate. As a control, migration toward fMLP (10⁻⁸ M) was evaluated. (A) Monocytes were mock infected or incubated at an MOI of 5 with both replication-competent TB40E and UV-inactivated TB40E (UV-TB40E). At 24 h after infection, the net numbers of cells that migrated were obtained by subtracting the number of cells that migrated in response to medium alone from the number of cells that migrated in response to chemokines. Net migration values (mean ± SD) of 10 experiments performed with monocytes from 10 different donors are shown. , P ≤ 0.05 between mock- and TB40E-infected cells. (B) Monocytes were infected with TB40E at different MOIs (5, 1, and 0.5 PFU/cell). At 24 h after infection, chemotaxis induced by CCL5 and CXCL12 was assessed. Values are means of three separate experiments. (C) In four different donors, the numbers of migrating monocytes were evaluated at different time points during HCMV infection. Values are the mean ± SD of four independent experiments; , P ≤ 0.05 between mock- and TB40E-infected cells.

Inline graphic — TB40E induces the block of chemokine-driven migration in monocytes. Monocyte chemotaxis toward inflammatory (CCL5, CCL2, and CX3CL1) and homeostatic (CCL19 and CXCL12) chemokines was evaluated with a Boyden chamber as described in Materials and Methods. The migration induced by the indicated chemokines (100 ng/ml) was evaluated by assessing each stimulus in triplicate. As a control, migration toward fMLP (10⁻⁸ M) was evaluated. (A) Monocytes were mock infected or incubated at an MOI of 5 with both replication-competent TB40E and UV-inactivated TB40E (UV-TB40E). At 24 h after infection, the net numbers of cells that migrated were obtained by subtracting the number of cells that migrated in response to medium alone from the number of cells that migrated in response to chemokines. Net migration values (mean ± SD) of 10 experiments performed with monocytes from 10 different donors are shown. , P ≤ 0.05 between mock- and TB40E-infected cells. (B) Monocytes were infected with TB40E at different MOIs (5, 1, and 0.5 PFU/cell). At 24 h after infection, chemotaxis induced by CCL5 and CXCL12 was assessed. Values are means of three separate experiments. (C) In four different donors, the numbers of migrating monocytes were evaluated at different time points during HCMV infection. Values are the mean ± SD of four independent experiments; , P ≤ 0.05 between mock- and TB40E-infected cells.