TABLE 1.
Quantitative assessment of viral binding to microtubules
| Condition(s) | % Capsid in pellet | Pa |
|---|---|---|
| MAP-dependent binding | ||
| MAP-containing cell lysate + microtubules | 74 ± 3 | Control |
| MAP-containing cell lysate + microtubules + 500 mM NaCl | 1.2 ± 0.2 | <0.01 |
| MAP-depleted cell lysate + microtubules | 27 ± 7b | <0.01 |
| MAP-depleted cell lysate + microtubules + Tau | 23 ± 8b | <0.01 |
| Anticytoplasmic dynein-depleted cell lysate + microtubules | 26 ± 7 | <0.01 |
| Irrelevant immunoglobulin G-depleted cell lysate + microtubules | 73 ± 3 | >0.1 |
| MAP-containing cell lysate prepared in 10 mM ATP + microtubules | 47 ± 3 | <0.01 |
| MAP-containing cell lysate prepared in 10 mM AMP-PNP + microtubules | 77 ± 7 | >0.1 |
| Competition studies | ||
| Cy3-AAV2 + naive Sf9 cell lysate | 90 ± 4 | Control |
| Cy3-AAV2 + pAAV2-Sf9 cell lysate | 1 ± 2 | <0.01 |
| Cy3-AAV2 + pNull-Sf9 cell lysate | 91 ± 3 | >0.1 |
| Cy3-AAV5 + naive Sf9 cell lysate | 95 ± 5 | Control |
| Cy3-AAV5 + pAAV2-Sf9 cell lysate | 1 ± 1 | <0.01 |
| Cy3-AAV5 + pNull-Sf9 cell lysate | 97 ± 1 | >0.1 |
| Cy3-AAVrh10 + naive Sf9 cell lysate | 95 ± 3 | Control |
| Cy3-AAVrh10 + pAAV2-Sf9 cell lysate | 1 ± 0.4 | <0.01 |
| Cy3-AAVrh10 + pNull-Sf9 cell lysate | 95 ± 3 | >0.1 |
| Cy3-adenovirus serotype 5 + naive Sf9 cell lysate | 68 ± 6 | Control |
| Cy3-adenovirus serotype 5 + pAAV2-Sf9 cell lysate | 3 ± 1 | <0.01 |
| Cy3-Adenovirus serotype 5 + pNull-Sf9 cell lysate | 63 ± 5 | >0.1 |
a
Control, condition followed by P values for comparator conditions.
b
P > 0.1 for comparison of these two values.