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. 2006 Aug;80(15):7339–7353. doi: 10.1128/JVI.00309-06

FIG. 5.

FIG. 5.

His-tagged protein affinity pull-down analysis of the region of USF1 that interacts with IE62. (A) E. coli-expressed full-length and truncated His-USF1 proteins were coupled to Ni-nitrilotriacetic acid magnetic agarose beads and incubated with recombinant IE62 protein. His-RPA14 was coexpressed with RPA32 in E. coli and used as a negative control. The binding of IE62 was examined by immunoblotting (upper panel). Coomassie blue staining shows the levels of the bound His-tagged proteins present on the beads (lower panel). (B) His-USF protein pull-down assays using purified bacterially expressed GST-IE62 (1-299) fusion protein. The binding of GST-IE62 (1-299) was examined by immunoblotting with anti-GST antibody (upper panel). Coomassie blue staining shows the levels of the bound His-tagged proteins present on the beads (lower panel).