FIG. 4.
Level of Wee1 kinase is stabilized in HeLa cells arrested in G2 following coexpression of the E4-17K and E4-16K proteins. Wee1 protein expression in synchronous HeLa cells infected with rAds expressing E4-17K or E4-16K or coinfected with both viruses 0 to 28 h (A) or 10 to 32 h (B) after release from a double thymidine block. Graphical representation of cell cycle progression is shown in a, c, and e. Western blot analysis of Wee1 levels, with β-actin acting as a protein loading control, is shown in b, d, and f.