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. 2006 Aug;80(15):7416–7426. doi: 10.1128/JVI.00196-06

FIG. 5.

FIG. 5.

Depletion of Wee1 by siRNA inhibits G2 arrest induced by E4-17/16K protein expression. (A) Western blot analysis of the Wee1 protein in HeLa cells either mock transfected or transfected with siRNAs specific for Wee1 (siWee1) or Luciferase (siLuciferase), followed by mock infection or infection with rAds expressing HPV1 E4 proteins or β-galactosidase. Expression of E4 was confirmed in the appropriate infections, and β-actin acted as a protein loading control. (B) The histogram shows G2+M:G1 ratios of data from four independent experiments, shown as means ± standard deviations. The single asterisk indicates a significant (99.9%) decrease in the G2+M:G1 ratio of siWee1-transfected E4-17/16K cells compared to that of mock-transfected (no siRNA) or siLuciferase-transfected E4-17/16K cells. The double asterisk indicates a significant (99.9%) G2 arrest in cells expressing the E4-16K or E4-17/16K protein compared to cells expressing β-galactosidase (no siRNA) or mock-infected cells (no siRNA). (C) Cell cycle profiles of E4-17/16K-expressing cells and control cells treated with siLuciferase or siWee1.