TABLE 1.

Trp⁸ fluorescence lifetimes and amplitude fractions in Bc-Csp

α1	τ1 (ns)	α2	τ2 (ns)	χ2*	Acrylamide† (M)
0.29 ± 0.06	2.45 ± 0.03	0.71 ± 0.06	6.16 ± 0.14	1.36	0.00
0.27 ± 0.04	1.86 ± 0.05	0.73 ± 0.05	3.94 ± 0.10	1.22	0.05
0.28 ± 0.06	1.52 ± 0.09	0.72 ± 0.07	2.96 ± 0.12	1.30	0.10
0.25 ± 0.09	1.12 ± 0.10	0.75 ± 0.08	1.85 ± 0.09	1.59	0.20
0.27 ± 0.08	0.84 ± 0.11	0.73 ± 0.07	1.39 ± 0.15	1.61	0.30
0.26 ± 0.05	0.65 ± 0.08	0.74 ± 0.05	1.15 ± 0.11	1.45	0.40

^*The value that defines the correctness of the fit.

^†Quenching of Trp⁸ in Bc-Csp (10 μM) with increasing amounts of acrylamide.