Figure 4.

Embryo and Seed Development in Crosses Using Hypomethylated 4x A. thaliana.

(A) Mean weight of desiccated mature seeds, calculated as for Figure 1F. From left to right: 26.9 ± 0.8 μg (n = 4) and 19.6 ± 2.9 μg (n = 9).

(B) Germination rate. From left to right: 100% (n = 28) and 7% (n = 88).

(C) to (F) Comparisons of seed development. (C) and (E) show mature seeds. Bar = 1 mm. (D) and (F) show embryos at 8 DAP, either extruded (D) or in cleared seeds (F), photographed using differential interference contrast optics. Bars = 100 μm (D) and 50 μm (F).

(C) and (D) [4xMET1a/sAt × 4xMET1a/sAt].

(E) and (F) [4xMET1a/sAt × 4xAa].