TABLE 1.

Exclusion of alcohols from condensed DNA arrays

DNA	Alcohol	λ, Å	Πexcess(27Å)/Π0	ΔNw, per basepair
Na+-DNA	MPD	4.0 ± 0.2	0.65 ± 0.02	42 ± 3
Co3+-DNA	MPD	3.65 ± 0.35	0.46 ± 0.02	34 ± 5
Spd3+-DNA	MPD	3.55 ± 0.25	0.49 ± 0.02	35 ± 4
	Δ(C-O) = 3*	3.6 ± 0.2	0.41 ± 0.02	32 ± 3
	Δ(C-O) = 2†	3.7 ± 0.2	0.28 ± 0.02	25 ± 3
	Δ(C-O) = 1‡	3.65 ± 0.25	0.22 ± 0.02	20 ± 3
	Methanol	4.45 ± 0.5	0.13 ± 0.03	11 ± 4
	Ethylene glycol	–	0.05 ± 0.01
	Glycerol	–	0.00 ± 0.02
	Threitol	0.95 ± 0.25	−0.10 ± 0.03
	Sorbitol	1.25 ± 0.15	−0.37 ± 0.04

The data shown in Figs. 2–4 were fit to an exponential function to determine an apparent decay length and best fit amplitude at 27 Å. The total number of excess waters was determined by integrating the exponential fit for Π_excess/Π₀ from D_int = ∞ to 20.9 Å (the spacing measured for dried Spd³⁺-DNA arrays) using Eqs. 3 and 5 and dV = √3 l D_int dD_int, where V is the water volume/basepair in the DNA phase and l is the rise/basepair assumed 3.4 Å.

^*An average of the data for 1-butanol, 2-butanol, and t-butanol.

^†An average of the data for 1-propanol, 2-propanol, 1,4-butanediol, and 2,3-butanediol.

^‡An average of the data for ethanol, 1,3-propanediol, and 1,2-propanediol.