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. 2006 Apr 14;91(3):873–881. doi: 10.1529/biophysj.106.082115

FIGURE 1.

FIGURE 1

Translocation of NBD-DMPE in POPC bilayers at 35°C. At given times of incubation (Inline graphic) at 35°C, the amount of NBD-DMPE in the outer monolayer was measured by its reaction with dithionite (A). The experimental curve was fitted with a mono-exponential and the recovered total concentration of NBD-DMPE (□), the concentration in the outer (▵) and in the inner (○) monolayer are presented in B. The best fit of a mono-exponential to the time-dependence of the concentration of NBD-DMPE in the outer and inner monolayers is also shown (Inline graphic = 2.0 × 10−4 s−1). The data can also be analyzed as the fraction of NBD-DMPE in the outer monolayer (C).