FIG. 6.
Virus assembly in NSP2*-complemented cells. MA104 cells were transfected with either IR (A) or g8D siRNA (B and C) and with a vector expressing NSP2* (C) and then infected with vTF7.3 and the tsE strain. Cells were maintained in the presence of 35S-labeled amino acids and harvested ∼18 h p.i. Virus particles pelleted (p) from cell lysates were analyzed by CsCl centrifugation. Gradient fractions were analyzed for density and for protein content by electrophoresis. The titer of the virus in the fractions containing TLPs (*) was determined by IFF assay.