FIG. 3.
Strain-specific effects of Q185R mutation on PrPSc formation. (A) Conversion to 3F4-positive PrPSc (upper panels) and expression of Mo3F4, Q90R, Q97R, Q159R, Q167R, Q171R, Q185R, Q211R, Q216R, and Q222R (lower panels) were measured by Western blotting using 3F4 antibody. The 3F4 epitope was present in all these constructs. (B) Inhibitory effects of constructs that did not convert were determined by cotransfection with Mo3F4 at a 1:1 or 1:2 DNA ratio. The blots were probed with 3F4 antibody.