Skip to main content
. 2006 Aug;80(16):7816–7831. doi: 10.1128/JVI.00532-06

FIG. 4.

FIG. 4.

Analysis of the cleavage site between Nterm and NTPase. (A) Alignment of the amino acid sequence of the putative junction of the MNV Nterm and NTPase proteins with those of CV, MDV, NV, and SHV. Numbers correspond to the ORF1 polyprotein sequence of each virus, and asterisks indicate conserved amino acids. (B) Comparative analysis of the MNV ORF1 polyprotein processing products synthesized in vitro from full-length genomic clone p20.3 (lane 1) and its derivative, p20.3m341, carrying an Nterm-NTPase cleavage site mutation (341EG→341AG) (lane 3). Lanes 2 and 4, in vitro-radiolabeled proteins derived from clones pCINterm and pCINTPase, encoding predicted sequences of Nterm (aa 1 to 341) and NTPase (aa 342 to 705), respectively.