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. 2006 Aug;80(16):8225–8235. doi: 10.1128/JVI.00395-06

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Copyright © 2006, American Society for Microbiology

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FIG. 4. — Sedimentation analysis of Fv1(20-200). (A) Concentration distribution at sedimentation equilibrium for 16 μM Fv1(20-200) at 15,000 rpm. The curve is the best fit to the data, and the top panel shows the residuals to this fit. M_w, is the weight-averaged molecular weight derived from global fitting of all the data in thousands. (B) Analysis of Fv1(20-200) by sedimentation velocity. The data were produced from a sedimentation velocity experiment at 42,000 rpm, scanned every 300 s. The points are the measured data, and the lines are the best fit to the data using a C(S) size distribution function and f/f_o of 1.7. Residuals from the fit are shown above. (C) The C(S) function corresponding to the best fit in panel A. (D) C(M) function corresponding to the best fit to the data in panel B. The fit quality and residuals are comparable to the C(S) function.