TABLE 1.
Animal | Immunization route and immunogena | End point infected cell titer for strain (subgroup) determined byb:
|
MAb obtained | |||
---|---|---|---|---|---|---|
ELISA
|
Microneutralization
|
|||||
NL100 (A1) | NL199 (B1) | NL100 (A1) | NL199 (B1) | |||
Hamster | i.n. NL1700 | 1:781,250 | 1:781,250 | 1:1,250 | <1:50 | 1017 |
i.n./i.p. PIV3/FNL100 | 757 | |||||
Hamster | i.n. NL199 | 1:25,600 | 1:25,600 | 1:100 | 1:800 | 836 |
i.n. NL100 | 659 | |||||
i.p. adeno FNL100/FNL199 | 967 | |||||
i.p. soluble FNL100/FNL199 | ||||||
Mouse | i.n. NL199 | 1:312,500 | 1:312,500 | 1:100 | 1:200 | 338 |
i.n. PIV3/FNL100 | 234 | |||||
i.p. adeno FNL100/FNL199 | 224 | |||||
i.p. soluble FNL100/FNL199 | ||||||
Mouse | i.n. NL199 | 1:312,500 | 1:312,500 | 1:50 | 1:1,600 | 710 |
i.n. NL100 | 344 | |||||
i.p. adeno FNL100/FNL199 | 628 | |||||
i.p. soluble FNL100/FNL199 |
Immunization routes were intranasal (i.n.) and intraperitoneal (i.p.).
End point titers were determined as described in Materials and Methods.