FIG. 8.

Percentages of BrdU⁺ cells present among efferent lymph cells and PBMCs after intravenous pulses of BrdU. The percentage of BrdU-stained cells, as determined by flow cytometry, is plotted for the total live-cell population as well as within IgM⁺, CD4⁺, CD8⁺, and WC1⁺ cell subsets. Numbers above each group of bars indicate enrichment (n-fold) for BrdU⁺ cells within a subset relative to the frequency of BrdU⁺ cells within the whole cell population. (A and B) BrdU was injected on day 5 after infection or mock infection, and lymph was collected for staining after 14 h (black bars), 38 h (gray), and 62 h (white). (C) BrdU was injected into sham-cannulated sheep 43 on day 5 after infection and blood was collected 15 h (black bars) and 40 h (gray) later. (D) On day 15, blood was collected from sham-cannulated sheep 43 to evaluate residual BrdU⁺ cells (white bars): relative enrichments within subsets were 0.6 (IgM^hi), 0.8 (IgM^mod), 2.0 (CD4⁺), 1.9 (CD8⁺), and 2.0 (WC1⁺). BrdU was then injected on day 16, and blood was collected 15 h (black bars) and 39 h (gray) later.