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. 2006 Aug;80(16):8019–8029. doi: 10.1128/JVI.02164-05

FIG. 1.

FIG. 1.

Induction of Sp100 isoforms in response to IFN-β treatment. (A) Structural and functional domains of Sp100 isoforms. (B) Induction of ISG15 and ISG54 mRNA was assessed by semiquantitative RT-PCR analysis. HEp-2 cells were treated with 500 U/ml of IFN-β for 18 h before total cellular RNA was extracted. (C) Levels of Sp100 isoforms were analyzed by semiquantitative RT-PCR from the same samples. (D) Induction of Sp100 isoforms in different cell lines. Levels of GAPDH mRNA were used to control an equal amount of mRNAs. To control DNA contamination we used RNA samples without RT (-RT). Asterisks indicate positions of GAPDH primers. M, molecular size marker.