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. 2006 Aug;80(16):8019–8029. doi: 10.1128/JVI.02164-05

FIG. 3.

FIG. 3.

Accumulation of Sp100 isoforms in HEp-2, HEK293, and 293-S cells. Whole-cell extracts (A) or nuclear fractions (B) of replicate HEp-2, HEK293, and 293-S cell cultures were stimulated with 500 U/ml of IFN-β for 18 h or left untreated as a control. 293-S cells were transfected with 2 μg of pSG5 vector DNA or constructs expressing Sp100A (A), Sp100B (B), Sp100HMG (HMG), or Sp100C (C). Note that sumoylation of Sp100 proteins, indicated by asterisks, is preserved in whole-cell extracts but not in nuclear fractions. WB, Western blot.