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. 2006 Aug;80(16):7789–7798. doi: 10.1128/JVI.00600-06

FIG. 4.

FIG. 4.

Effects of mutations in the PA subunit on the assembly of the influenza virus RNA polymerase complex. (A) Expression and assembly of the polymerase subunit PB1, TAP-tagged PB2, and PA mutants in 293T cells. (B) Expression of TAP-tagged PA mutants alone in 293T cells. (C) Expression of PA mutants alone in total cell lysates of 293T cells. TAP-tagged proteins were partially purified from lysates of transfected 293T cells 24 h posttransfection with immunoglobulin G (IgG)-Sepharose, and bound proteins were cleaved by tobacco etch virus protease. The samples were analyzed by silver-stained 7.5% SDS-PAGE. (D1 and D2) Effects of the proteasome inhibitor on PA mutants. TAP-tagged PA was purified from cell lysates with IgG Sepharose and analyzed by 7.5% SDS-PAGE and silver staining. The positions of PB1, PB2-TAP, PA, and PA-TAP are shown on the right. The positions of marker proteins are indicated, in kilodaltons. WT, wild type.