TABLE 1.
Mutant PA activity
| Parameter | Mean mutant PA activity ± SD (%)a
|
||
|---|---|---|---|
| K102A | D108A | K134A | |
| Globin mRNA-primed transcription | 12 ± 2.1 | 4.1 ± 0.6 | 1.4 ± 0.7 |
| Capped RNA-primed transcription | 4.2 ± 0.8 | 71 ± 9.4 | 35 ± 9.4 |
| Endonuclease activity | 10 ± 0.5 | 0.1 ± 0.8 | 0.7 ± 0.4 |
| Cap-binding activity | 10 ± 1.0 | 44 ± 5.4 | 29 ± 6.1 |
| vRNA-binding activity | 49 ± 7.9 | 48 ± 9.7 | 33 ± 6.9 |
| Replication activity (vRNA) | 34 ± 5.4 | 38 ± 9.0 | 20 ± 3.5 |
| Replication activity (cRNA) | 54 ± 5.3 | 49 ± 4.6 | 23 ± 2.5 |
a
Data are expressed as percentages of wild-type activity from three independent experiments.